2008•Sichuan Medical JournalRequires access

Effect of Phenylacetate on the Expression of Bcl-2,Bax and Caspase-3 in Rat C_6 Glioma Cells

Chao You

Abstract

Objective To observe the effect of differentiation inducer phenylacetate(PA)on the expression of Bcl-2,Bax and Caspase-3 in rat C6 glioma cells and explore the possible mechanisms of PA action.Methods C6 cells were cultured in vitro.In experiment group,C6 cells were induced by PA at concentration of 5.0mmol/L for 72h.In control group,no PA was administrated.The expression of Bcl-2,Bax and Caspase-3 was detected by immunocytochemistry.Results The expression of Bcl-2 was unchanged in the two group(P0.05),while the expression of Bax and Caspase-3 in the experiment group was higher than that in the control group(P0.01).Conclusion PA can enhance the expression of Bax and Caspase-3,which might consequently induce apoptosis in C6 glioma cells.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Apoptosis, Phenylacetate, Medicine, Glioma, Immunocytochemistry, In vitro, Caspase 3, Molecular biology