2010•Zhongguo tangniaobing zazhiRequires access
The expression of hypoxia inducible factor-1α and hemo oxygenase-1 in renal tissue of diabetic rats
Yang Yi-bi
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Abstract
Objective To investigate whether hypoxic conditions exist and its relations to pathologic changes in the kidney of streptozotocin-induced diabetic rats.Methods Sprague-Dawley male rats were divided into diabetic nephropathy (DN) group and control group.Urinary albumin,blood sugar,serum urea and creatinine were monitored at 2,4,8,12 and 16 weeks.Renal pathologic changes and levels of hypoxia inducible factor-1α(HIF-1α) and hemooxygenaseT (HO-1) were observed at the same time points. Results HIF-1α and HO-1 in kidney were outstandingly expressed in renal tubules of the cortex or the close area between cortex and medulla,and their staining degree were significantly stronger in DN group than in the control group (P0.01),being maximal at 8 and 12 weeks.In DN group,a strong correlation between HIF-1α and HO-1 levels was found(r=0.838,P0.01),and their levels were correlated with urinary albumin,nephrohypertrophy index and glomerular volume.Conclusions Hypoxia in the diabetic kidney involves in development of diabetic nephropathy.