Effect leinoleic acid/docosahexaenoic acid on apoptosis of human gastric cancer cells

Objective To explore the effects of different constituent ratio leinoleic acid(LA)/docosahexaenoic acid(DHA) on apoptosis of human gastric cancer cell line MKN-45.Methods With uniform design,the better constituent ratio for the inhibition of MKN-45 cell was scrceend.Then the best ratio was determined with randomized block design.The tumor volume,side-effect,and tumor weigh of tumor-bearing nude mice after treatment were observed.The morphological changes of tumor tissue were assessed by HE staining.Results The LA/DHA constituent ratio of 1:3 showed maximum inhibition of the tumor cells.Six weeks after high concentration treatment,the mean tumor volume decreased by 21.97% and the mean tumor weigh by 18.09% with statistical significance compared with that of control group(P0.05).The toxicity of the treatment was not obvious.Microscopic examination showed that there were massive areas of cytoclasis and apoptosis in the tumor tissue of the high concentration group and 5-FU group.Conclusion The 1:3 constituent ratio of LA/DHA could induce the apoptosis of human gastric cancer cell line MKN-45.The high concentration of 1:3 constituent ratio treatment could also inhibit tumor in vivo without significant side-effect.