Protective effect of hyperoxic solution combined with ischemic postconditioning on hepatic injury induced by intestinal ischemia reperfusion in rabbits

Objective To observe the protective effect of hyperoxic solution combined with ischemic postconditioning( IP) on hepatic injury induced by intestinal ischemia reperfusion in rabbits. Methods A total of 48 rabbits were randomly divided into sham operation group( SO group),ischemia reperfusion group( IR group),hyperoxic solution group( HO group) and HO + IP group( HI group),with 12 rabbits in each group. The rabbits in SO group were treated with exposing superior mesenteric artery( SMA). The rabbits in IR group were treated with SMA interruption and 120 minutes reperfusion. The rabbits in HO group were treated with hyperoxic solution by intervenous drop infusion in the course of 60 minutes SMA interruption and 120minutes reperfusion. The rabbits in HI group were treated with hyperoxic solution by intervenous drop infusion before reperfusion,at the same time,three cyclical reperfusion( 30 seconds) and interruption( 30 seconds) were performed. The arterial blood,venous blood,enteric and hepatic tissue were collected at the time of 120 minutes after reperfusion. The levels of tumor necrosis factor-α( TNF-α),interleukin-10( IL-10),alanine aminotransferase( ALT),aspartate aminotransferase( AST) and endotoxin were detected. The level of malonaldehyde( MDA),the activity of myeloperoxidase( MPO) and superoxide dismutase( SOD) in enteric and hepatic tissue were detected. The intestinal mucosae injury was evaluated by Chiu-6 standard scoring. The bacterial metachoresis rat was observed by germiculture. The expression of nuclear factor-κB( NF-κB) in hepatic tissue was detected by immunohistochemistry. Results The levels of serum TNF-α,IL-10 and endotoxin in IR group were significantly higher than those in SO group( P 0. 01). The levels of serum TNF-α and endotoxin in HO group and HI group were significantly lower than those in IR group( P 0. 05). The levels of serum TNF-α and endotoxin in HI group were significantly lower than those in HO group( P 0. 05,P 0. 01). The IL-10 level in HO group and HI group was significantly higher than those in IR group( P 0.01). There was no significant difference in IL-10 level between HO group and HI group( P 0.05). The serum MPO activity and MDA level in IR group were significantly higher than those in SO group( P 0. 01),the serum MPO activity and MDA level in HO group and HI group were significantly lower than those in IR group( P 0. 01),and the serum MPO activity and MDA level in HI group were significantly lower than those in HO group( P 0. 05,P 0. 01). The serum SOD activity in IR group was significantly lower than those in SO group( P 0. 01),the serum SOD activity in HO group and IR group were significantly higher than those in IR group( P 0. 01),and the serum SOD activity in HI group was significantly higher than that in HO group( P 0. 01). The intestinal mucosae injury score of rabbits in HO group and HI group was significantly higher than that in SO group( P 0. 01). The intestinal mucosae injury score of rabbits in HO group and HI group was significantly lower than that in IR group( P 0. 01),and the intestinal mucosae injury score of rabbits in HI group was significantly lower than that in HO group( P 0. 01). The MPO activity and MDA level in intestinal mucosae tissues of rabbits in IR group were significantly higher than those in SO group( P 0. 01). The MPO activity and MDA level in intestinal mucosae tissues of rabbits in HO group and HI group were significantly lower than those in IR group( P 0. 01). The MPO activity and MDA level in intestinal mucosae tissues of rabbits in HI group were significantly lower than those in HO group( P 0. 01). The SOD activity in intestinal mucosae tissues of rabbits in IR group was significantly lower than that in SO group( P 0. 01). The SOD activity in intestinal mucosae tissues of rabbits in HO group and HI group was significantly higher than that in IR group( P 0. 01).The SOD activity in intestinal mucosae tissues of rabbits in HI group was significantly higher than that in HO group( P 0. 05). There was no bacterial metachoresis of hepatic tissue of rabbits in SO group. The bacterial metachoresis rat of hepatic tissue of rabbits was 100. 0% in IR group,while the bacterial metachoresis rat of hepatic tissue of rabbits was 83. 30% in HO group and HI group,but there was no significant difference in bacterial metachoresis rat of hepatic tissue of rabbits in HO,HI and IR group( P 0. 05). The NF-κB p65 positive expression in hepatic tissue of rabbits in IR group,HO group and HI group was significantly higher than that in SO group( P 0. 01); in HO group and HI group it was significantly lower than that in IR group( P 0. 01),and in HI group it was significantly lower than that in HO group( P 0. 05). The MPO activity and the levels of MDA,ALT,AST in hepatic tissue of rabbits in IR group were significantly higher than those in SO group( P 0. 01). The MPO activity and the levels of MDA,ALT,AST in hepatic tissue of rabbits in HO group and HI group were significantly lower than those in IR group( P 0. 01). The MPO activity and the levels of MDA,ALT,AST in hepatic tissue of rabbits in HI group were significantly lower than those in HO group( P 0. 05). The SOD activity in hepatic tissue of rabbits in IR group was significantly lower than that in SO group( P 0. 01),the SOD activity in hepatic tissue of rabbits in HO group and HI group was significantly higher than that in IR group( P 0. 01),and in HI group it was significantly higher than that in HO group( P 0. 05).Conclusion Hyperoxic solution combined with IP can relieve the hepatic injury induced by intestinal ischemia reperfusion in rabbits.