Cytological observations on male sterile anther and pollen developments induced by chemical hybridizing agent GENESIS in wheat

GENESIS is a brand name given to a new chemical hybridizing agent (CHA) that has been devel-oped by Monsanto Company, USA. At the rate of 5.0 kg/hm~2, with application active window from Feeke’s stage 8.0 to 9.0 (development stage of young spike from formation of anther chamber to quadrant), GENESIS could induce male sterility rate 100%. GENESIS had a good effect on male sterile inducing of common wheat (Triticum aestivm L. ). The development of anther and microsporogenesis of male sterility by GENESIS were studied in comparison with untreated controls. The results of it as follows: pollen aborting in five types might occur in later stage of microsporgenesis. Mononuclear pollen, binuclear pollen, abnormal trinuclear, polynuclear pollen and empty pollen were found in anther cell. This is due to the deviation from normal development mode of tapetum in wheat induced by GENESIS and rise to abnormal tapetum. The time of the abnormal tapetum dissociation was delayed.