cDNA microarray to identify cell proliferation and cell cycle genes associated with benzene poisoning

Objective To detect cell proliferation and cell cycle genes associated with benzene poisoning by using gene expression profile analysis.(Methods)Peripheral white blood cell gene expression profile of 7 benzene poisoning patients,including one aplastic anemia,was determined by microarray.Seven chips from normal workers were served as control.Cluster analysis of gene expression profile was performed.Differentially expressed genes associated with benzene poisoning were determined.(Results) Among the 338 target genes,40 genes differentially expressed were identified,including 17 up-regulated genes,mainly,IFIM1、CDKN1B、RBL2、GSPT1、FOXO1Aetc,and 19 down-regulated genes,mainly,CDC37、MDM4、EVI5、SKP2 etc,and other 6 genes expression showing difference.Cluster analysis showed that the expression profiles of 40 genes were associated with benzene poisoning.Conclusion Differentially pathways may play a important role in the pathogenesis of benzene poisoning.