Simultaneous analysis of five phenolic compounds in fructus elaeagni by high performance liquid chromatography

A method had been developed for the simultaneous determination of the contents of chlorogenic acid,caffeic acid,ferulic acid,coumalic acid and cinnamic acid in fructus elaeagni by high performance liquid chromatography.After being extracted by acetonitrile from samples,five phenolic compounds were separated on a Hypersil ODS 100×4.6mm,5μm column using acetonitrile and 0.6% orthophosphoric acid as mobile phase with linear gradient elution,and detected by photodiode array detector(PDAD at wavelength of 323nm and 280nm.There were good linear correlations between the concentrations and peak areas of the five components with the correlation coefficients of 0.991 ~ 0.997.The average recoveries were 93.3% ~ 98.1% with relative standard deviations of 3.1% ~ 7.4%(n=6).The results suggested that the content of chlorogenic acid,caffeic acid,ferulic acid,coumalic acid and cinnamic acid in fructus elaeagni was 0.472,0.373,0.425,0.529,0.381mg/kg respectively.