ScholarLens Back to search
2008•Harbin Yike Daxue xuebaoRequires access

Isolation,culture and identification of adipose-derived stem cell in rats

Yuling Jiang

Open publisher page 0 citations

Abstract

Objective To obtain adipose-derived stem cell(ADSC)of rats.Methods ADSCs of Wistar rats were digested with collagenase typeⅠsolution.The growth curve of ADSCs was checked by cell counting;The cell proliferation was tested by MTT method;The molecular markers of ADSCs were detected with immunofluorescence.Results The morphology of ADSCs was identical to fibroblast-like and the cells showed active proliferative ability.Vimentin and CD105 antigens could be detected,but CD31 and α-SMA antigens were negative.Conclusion ADSCs can be used as seed cells for tissue engineering due to the simple isolation and stable,active growth of ADSCs.

About this research paper

What this paper is about

Objective To obtain adipose-derived stem cell(ADSC)of rats.Methods ADSCs of Wistar rats were digested with collagenase typeⅠsolution.The growth curve of ADSCs was checked by cell counting;The cell proliferation was tested by MTT method;The molecular markers of ADSCs were detected with immunofluorescence.Results The morphology of ADSCs was identical to fibroblast-like and the cells showed active proliferative ability.Vimentin and CD105 antigens could be detected,but CD31 and α-SMA antigens were negative.Conclusion ADSCs can be used as seed cells for tissue engineering due to the simple isolation and stable,active growth of ADSCs.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To obtain adipose-derived stem cell(ADSC)of rats.Methods ADSCs of Wistar rats were digested with collagenase typeⅠsolution.The growth curve of ADSCs was checked by cell counting;The cell proliferation was tested by MTT method;The molecular markers of ADSCs were detected with immunofluorescence.Results The morphology of ADSCs was identical to fibroblast-like and the cells showed active proliferative ability.Vimentin and CD105 antigens could be detected,but CD31 and α-SMA antigens were negative.Conclusion ADSCs can be used as seed cells for tissue engineering due to the simple isolation and stable,active growth of ADSCs.

Key concepts: Collagenase, Adipose tissue, Stem cell, Cell culture, Mesenchymal stem cell, Antigen, CD31, Biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Isolation,culture and identification of adipose-derived stem cell in rats — Research Paper | ScholarLens