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The RPS3a level in the process of cellular apoptosis: II. Preparation and purification of polyclonal antibody to GST/S3a

Sun Aping

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Abstract

Objective: To prepare and purify polyclonal antibody to GST/S3a. Methods: The antibody was extracted by salting method ,the IgG was purified with protein A-agarose column and the polyclonal antibody was detected by ELISA. Results: The acitivity of the polyclonal antibody to GST/S3a was normal which showed the production of producing antigen-antibody response compound. Conclusions: The prepared antibody serum has the activity to the fusion protein GST/S3a, which provides a base for the next step.

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What this paper is about

Objective: To prepare and purify polyclonal antibody to GST/S3a. Methods: The antibody was extracted by salting method ,the IgG was purified with protein A-agarose column and the polyclonal antibody was detected by ELISA. Results: The acitivity of the polyclonal antibody to GST/S3a was normal which showed the production of producing antigen-antibody response compound. Conclusions: The prepared antibody serum has the activity to the fusion protein GST/S3a, which provides a base for the next step.

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Available abstract

Objective: To prepare and purify polyclonal antibody to GST/S3a. Methods: The antibody was extracted by salting method ,the IgG was purified with protein A-agarose column and the polyclonal antibody was detected by ELISA. Results: The acitivity of the polyclonal antibody to GST/S3a was normal which showed the production of producing antigen-antibody response compound. Conclusions: The prepared antibody serum has the activity to the fusion protein GST/S3a, which provides a base for the next step.

Key concepts: Polyclonal antibodies, Antibody, Agarose, Molecular biology, Chemistry, Chromatography, Biology, Immunology

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The RPS3a level in the process of cellular apoptosis: II. Preparation and purification of polyclonal antibody to GST/S3a — Research Paper | ScholarLens