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1-deoxymannojirimycin induced endoplasmic reticulum stress in hepatocellular carcinoma cell line 7721

Zonghou Shen

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Abstract

Purpose To study the relation between the inhibition of Golgi α-1,2mannosidase Ⅰby 1-deoxymannojirimycin(DMJ) and endoplasmic reticulum stress(ER stress) in hepatocellular cell line SMMC7721. Methods SMMC7721 cells were treated with DMJ.Then the key molecules involving in ER stress such as glucose regulated protein 78(GRP78),X box binding protein 1(XBP1),C/EBP homologus protein(CHOP) and caspase12 was analyzed on mRNA or protein levels with RT-PCR or Western blot. Results GRP78 expression on both mRNA and protein levels were upregulated,the spliced forms of XBP1 mRNA and relative protein were found,CHOP protein was increased and caspase-12 was activated in SMMC7721 with DMJ treatment. Conclusions These results suggested that DMJ could induce cellular ER stress in SMMC7721.

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Purpose To study the relation between the inhibition of Golgi α-1,2mannosidase Ⅰby 1-deoxymannojirimycin(DMJ) and endoplasmic reticulum stress(ER stress) in hepatocellular cell line SMMC7721. Methods SMMC7721 cells were treated with DMJ.Then the key molecules involving in ER stress such as glucose regulated protein 78(GRP78),X box binding protein 1(XBP1),C/EBP homologus protein(CHOP) and caspase12 was analyzed on mRNA or protein levels with RT-PCR or Western blot. Results GRP78 expression on both mRNA and protein levels were upregulated,the spliced forms of XBP1 mRNA and relative protein were found,CHOP protein was increased and caspase-12 was activated in SMMC7721 with DMJ treatment. Conclusions These results suggested that DMJ could induce cellular ER stress in SMMC7721.

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Available abstract

Purpose To study the relation between the inhibition of Golgi α-1,2mannosidase Ⅰby 1-deoxymannojirimycin(DMJ) and endoplasmic reticulum stress(ER stress) in hepatocellular cell line SMMC7721. Methods SMMC7721 cells were treated with DMJ.Then the key molecules involving in ER stress such as glucose regulated protein 78(GRP78),X box binding protein 1(XBP1),C/EBP homologus protein(CHOP) and caspase12 was analyzed on mRNA or protein levels with RT-PCR or Western blot. Results GRP78 expression on both mRNA and protein levels were upregulated,the spliced forms of XBP1 mRNA and relative protein were found,CHOP protein was increased and caspase-12 was activated in SMMC7721 with DMJ treatment. Conclusions These results suggested that DMJ could induce cellular ER stress in SMMC7721.

Key concepts: Endoplasmic reticulum, XBP1, Unfolded protein response, Glucose-regulated protein, CHOP, Golgi apparatus, Downregulation and upregulation, Western blot

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