Transfection and expression of the recombinant adeno-associated virus carrying human interferon-γ in human lymphocyte

Objective In order to study the transfection and expression of the recombinant pWIG of Adeno associated virus and Human interferon γ(HuIFN γ) in human lymphocyte.Methods After transfecting pWIG into human lymphocyte via calcium phosphate, DNA, RNA and protein extracted from transfected cells were detected by polymerase chain reaction(PCR), reverse transcription PCR(RT PCR) and ELISA. Results The 504 bp bands were detected from PCR and RT PCR products of the DNA and RNA from the cells transfected, and HuIFN γ protein were detected to be 17.2 pg/3×10 6 cells by ELISA.Conclusion The recombinant pWIG could produce HuIFN γ mRNA after the transfection via calcium phosphate, and translate corresponding protein further. This lays ground for the further research of gene therapy.