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DPPH Radical Scavenging Effect of Compound Tangmaikang

Zhang Yi

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Abstract

Objective:To establish a method of scavenging DPPH radical,and then evaluate DPPH radical scavenging effect of compound Tangmaikang.Method: Investigated the reaction time,temperature,the concentration of DPPH and the extraction solvent and method,the study on the DPPH radical scavenging effect of 14 batches Tangmaikang and drug-containing serum was carried out.Result: Tangmaikang was extracted with 60% methanol by ultrasonic for 30 min.The optimum reaction conditions were obtained as follows:DPPH concentration 0.099 8 g·L-1,reaction temperature 60 ℃,reaction time 50 min.The IC50 of 14 batches of Tangmaikang was 0.48 mg.The DPPH clearance of drug-containing serum was lower than blank serum.Conclusion: Tangmaikang has antioxidant activity in vitro.

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What this paper is about

Objective:To establish a method of scavenging DPPH radical,and then evaluate DPPH radical scavenging effect of compound Tangmaikang.Method: Investigated the reaction time,temperature,the concentration of DPPH and the extraction solvent and method,the study on the DPPH radical scavenging effect of 14 batches Tangmaikang and drug-containing serum was carried out.Result: Tangmaikang was extracted with 60% methanol by ultrasonic for 30 min.The optimum reaction conditions were obtained as follows:DPPH concentration 0.099 8 g·L-1,reaction temperature 60 ℃,reaction time 50 min.The IC50 of 14 batches of Tangmaikang was 0.48 mg.The DPPH clearance of drug-containing serum was lower than blank serum.Conclusion: Tangmaikang has antioxidant activity in vitro.

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Available abstract

Objective:To establish a method of scavenging DPPH radical,and then evaluate DPPH radical scavenging effect of compound Tangmaikang.Method: Investigated the reaction time,temperature,the concentration of DPPH and the extraction solvent and method,the study on the DPPH radical scavenging effect of 14 batches Tangmaikang and drug-containing serum was carried out.Result: Tangmaikang was extracted with 60% methanol by ultrasonic for 30 min.The optimum reaction conditions were obtained as follows:DPPH concentration 0.099 8 g·L-1,reaction temperature 60 ℃,reaction time 50 min.The IC50 of 14 batches of Tangmaikang was 0.48 mg.The DPPH clearance of drug-containing serum was lower than blank serum.Conclusion: Tangmaikang has antioxidant activity in vitro.

Key concepts: DPPH, Chemistry, Scavenging, Methanol, Antioxidant, Solvent, Organic chemistry, Nuclear chemistry

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