Hippocampus Amino Acid Is Involved In Regulating the Learning and Memory and C-fos Expression within Hippocampus CA1、CA3 in the Fatigue Training Rats

Objective:Observing the influence of fatigue training on learning and memory,Hippocampal amino acid content and c-fos expression in the Hippocampus CA1,CA3 of the fatigue training rats and inquiring into the mechanism of the fatigue training decreasing learning and memory. Methods :30 SD rats(3 months)were chosen and randomly divided into 3 groups,which included control group(C),aerobic training group(AT)and fatigue training group(FT). The C group rats kept normal activities,AT group rats undertook a 8-week moderate-load treadmill training and FT group rats undertook a 8-week fatigue training with progressively increasing load. The morris water maze test was used to assess each animal's capability of learning and memory the next day after 8-week treadmill training,then hippocampal amino acid content were examined,c-fos expression in hippocampus CA1,CA3 was detected and analyzed by using ABC immuneohistochemical technique and semi quantitative. Results:(1)Compared with the C group,Glu content in hippocampal significantly increased(P0.05,P0.01)in the AT and FT group rats;GABA content in hippocampal was not significant difference(P0.05)and the ratio of Glu/GABA increased in AT group rats;GABA content and the ratio of Glu/GABA in hippocampal significantly increased(P0.05,P0.01)in FT group rats when compared to the C group rats(.2)Compared with the C group,escape latency shortened(P0.05),the times across platform increased(P0.05)in AT group rats,but escape latency extened(P0.01)and the times across platform decreased(P0.05)in FT group rats;(3)Compared with the C group,the number and area of cfos in hippocampus CA1,CA3 notably increased in the AT and FT group rats(P0.05,P0.01). Conclusion:Glu over secretion in hippocampus causing c-fos over expression in hippocampus CA1,CA3 may contribute to the learning and memory declining in fatigue training rats.