The relationship between CBR2 Activation and Injury Induced by Inflammation

Objective:To investigate the effects of cannabinoid CBR2 receptor agonist AM1241 preconditioning on microglia activation and injury induced by lipopolysaccharide(LPS) plus interferon-γ(IFN-γ).Methods:LPS plus IFN-γ was used to induce inflammation.MTT assay was used to find a suitable AM1241 concentration for preconditioning.Cells were pretreated with medium containing different AM1241 concentrations,from 0μM to 10μM;5μM was chosen for next steps.Then cells were assigned to control group,AM1241 group,LPS/IFN-γ group and AM1241+LPS/IFN-γ group.After AM1241 pretreatment,the medium of 4 groups were changed with normal medium.2h later,the medium of Control and AM1241 groups were changed with normal medium again and cul-tured for 24h.The medium of LPS/IFN-γ and AM1241+ LPS/IFN-γ groups were changed with medium containing LPS and IFN-γ.Cells were cultured for 24h.Microglial metabolism was assessed by MTT assay;NO release was measured by Reagent Kit;The concen-trations of inflammatory factors(TNF-α,IL-1β and IL-10) were detected by enzyme linked immunosorbent assay reagent kit(ELISA);Microglial shapes were observed through microscope.Results:cell metabolism of AM1241 group was higher than that of the Control group significantly(P0.05);AM1241 group released less NO,TNF-α,IL-1β and IL-10 than that of LPS/IFN-γ group(P0.05).Conclusion:Cannabinoid CBR2 receptor agonist AM1241 preconditioning reduces microglial activation and injury induced by inflam-mation.