Change and Significance of T cell subsets chronic HBV infected patients

Objective The data of CD4+, CD8+ T cell subsets and HBV DNA were analyzed,To study the change and significance of T cell subsets in chronic HBV infected patients.Methods CD4 and CD8+T cell subsets in peripheral blood mononuclear cells was determined with flow cytometry. The fluroescent quantitative PCR was used to detected the HBV DNA. ELISA kits were used to assay the HBeAg and anti_HBe in serum.Results The number of CD4 T cell in patients with liver cirrhosis (499.75±304.46) and hepatic carcinoma (296.59±279.51) were significantly lower than that of acute hepatitis patients (682.21±299.74,P0.05).The level of CD8+ T cells in patients with liver cirrhosis(303.45±225.84) and hepatic carcinoma(296.59±279.51) were obviously lower than that of patients with acute hepatitis (542.83±277.35,P0.05). There was no difference between the ntt number of CD4+ T cells in 59 cirrhotic patients with anti-HBe positive (477.42±206.39) and those of 35 patients with HBeAg positive (512.37±261.87), however; the number CD8+ T cells of the former group was significantly higher than that of the latter group (was 277.81±212.58;P=0.41); HBV DNA load in sera of cirrhotic patients and hepatic carcinoma patients was remarkable lower than that of chronic severe hepatitis (P=0.05 ).The value of HBV DNA between the group of hepatic carcinoma and cirrhosiswas similar (P= 0.05).Conclusion The level of CD4+ and CD8+ T cells in the group of cirrhosis and hepatic carcinoma are significantly lower than that of acute hepatitis and health positive control. HBV positive patieats with the number of CD8+ T cell being remarkable lower should be excluded from the possible existence of liver cancer.