Antibiotic-resistance testing and PFGE typing analysis of Shigella strains isolated from Gansu province(2006-2009)

Objective To study the antibiotic-resistance and pulse-field gel electrophoresis(PFGE)type of Shigella strains isolated from Gansu province in 2006-2009.Methods 470 strains of Shigella isolated from Gansu province in 2006-2009 were identified by biochemistry and sero-typing methods,and antibiotic-resistance and molecular typing were tested using PFGE and K-B methods.Results Shigella flexneri was still the predominant serogroup in Gansu province;serotype Fxv isolates accounted for most of the Shigella flexneri strains(44.5%),followed by 2a serotype(33.1%).All the S.flexneri and S.sonnei strains were resistant to Ampicillin,Tetracycline and Rifampicin(90.3-100%).CN0200 was the predomiant PFGE pulsetype of Shigella flexneri;S.flexneri strains with the same serotype having the simillar PFGE pulsetypes,and could be classified to one group.Conclusions The serotype,antibiotic-resistance and PFGE character of Shigella strains isolated from Gansu province were demonstrated in this study.The results will play a key role in clinical treatment,especially in preventing and controlling bacterial dysentery in Gansu and China.