Effects of all-trans retinoic acid on the human ovarian cancer HO8910 cell line

Objective:To investigate the effects of all-trans retinoic acid on the human ovarian cancer cell line HO8910.Methods:The human ovarian cancer cell line HO8910 cultured in vitro were treated with various concentrations of all-trans retinoic acid.The cell proliferation inhibition was detected by MTT assay;The morphologic changes of cell differentiation were observed under inverted microscope;The invasion capability was evaluated by transwell chambers.Results:1.ATRA of 10-7～10-5mol/L inhibited the proliferation of HO8910 cell significantly in a dose-dependent and time-dependent manner(P0.05)and its IC50 were 2.331×10-5 mol/L、0.998×10-6 mol/L、0.891×10-7 mol/L at 24 hours、48 hours and 72 hours,separately.2.Treated by 10-6 mol/L ATRA,HO8910 cells showed striking morphological characteristics of differentiation under microscope.3.The number of invasive cells decreased significantly after treated by 10-6 mol/L ATRA(P0.05).Conclusion:ATRA can both inhibit the proliferation and invasion of human ovarian cancer cell line HO8910,Which expect to provide an effective and novel approach for clinical treatment of human ovarian cancer.