ScholarLens Back to search
2006•Chongqing yixueRequires access

Culture and differentiation of endothelial progenitor cells from peripheral blood in vitro

Zhaohua Geng

Open publisher page 0 citations

Abstract

Objective To investigate the isolation,culture,proliferation and differentiation of endothelial progenitor cells(EPCs) from human circulating blood in vitro.Methods The mononuclear cells were obtained by Ficoll density gradient centrifugation.The isolated cells were suspended in Medium 199 with 20% fetal blood serum(FBS) and vascular endothelial growth factor(VEGF) for culturing,differentiating and proliferating.The expressions of specific antigens on cell surface were analysed by immunohistochemistry,immunocytochemistry and fluorescence-activated cell sorting(FACS).Results The culture cells were positively expressed for vwF,CD31 and CD34 and could be shown to endocytose ac-LDL and UEA-I.Conclusion EPCs can be obtained from mononuclear cells in peripheral blood and be differentiated into endothelial cells in vitro.

About this research paper

What this paper is about

Objective To investigate the isolation,culture,proliferation and differentiation of endothelial progenitor cells(EPCs) from human circulating blood in vitro.Methods The mononuclear cells were obtained by Ficoll density gradient centrifugation.The isolated cells were suspended in Medium 199 with 20% fetal blood serum(FBS) and vascular endothelial growth factor(VEGF) for culturing,differentiating and proliferating.The expressions of specific antigens on cell surface were analysed by immunohistochemistry,immunocytochemistry and fluorescence-activated cell sorting(FACS).Results The culture cells were positively expressed for vwF,CD31 and CD34 and could be shown to endocytose ac-LDL and UEA-I.Conclusion EPCs can be obtained from mononuclear cells in peripheral blood and be differentiated into endothelial cells in vitro.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To investigate the isolation,culture,proliferation and differentiation of endothelial progenitor cells(EPCs) from human circulating blood in vitro.Methods The mononuclear cells were obtained by Ficoll density gradient centrifugation.The isolated cells were suspended in Medium 199 with 20% fetal blood serum(FBS) and vascular endothelial growth factor(VEGF) for culturing,differentiating and proliferating.The expressions of specific antigens on cell surface were analysed by immunohistochemistry,immunocytochemistry and fluorescence-activated cell sorting(FACS).Results The culture cells were positively expressed for vwF,CD31 and CD34 and could be shown to endocytose ac-LDL and UEA-I.Conclusion EPCs can be obtained from mononuclear cells in peripheral blood and be differentiated into endothelial cells in vitro.

Key concepts: Progenitor cell, Peripheral blood mononuclear cell, CD31, Ficoll, CD34, Cell sorting, In vitro, Endothelial stem cell

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Culture and differentiation of endothelial progenitor cells from peripheral blood in vitro — Research Paper | ScholarLens