2014•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access
Effect of deguelin on viability and apoptosis of human cervical cancer Hela cells
XU Xi-zhon
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Abstract
【Objective】To investigate the effect of deguelin on viability and apoptosis of human cervical cancer HeLa cell. 【Methods】 Using different concentrations(0, 10, 100, 500, 1 000 nmol/L) of deguelin on HeLa cells at 24 h, 48 h, 72 h. SRB assay was used to detect the cell viability of each group at different times; Hoechst 33258 staining assay was used to observe the morphological changes induced by deguelin; Annexin V-FITC double labeled flow cytometry was used to detect the apoptosis; the cell cycle was analyzed via flow cytometry. 【Results】 The viability of HeLa cells was inhibited by deguelin in a time and dose dependent manner. The flow cytometry results showed that deguelin could induce HeLa cells apoptosis and the cell cycle was arrested in G0/G1 phase. 【Conclusion】 Deguelin can efficiently inhibit the viability and induce apoptosis of Hela cells in vitro.