The observation of PSD-95 immunohistochemistry in cerebral regions of morphine-withdrawal rats in different dependent time

Objective To evaluate the effect of PSD-95 on morphine withdrawal by observation postsynaptic density protein-95(PSD-95) immunoreactivity in cerebral regions of morphine-withdrawal rats in different dependent time.Methods Rat models of morphine dependent 1 week,2 weeks and 4 weeks was established by morphine inject into peritoneal cavity.After natural withdrawal of defendant rats,immunoreactivity for PSD-95 in hippocampus,neostriatum,amygdaloid body,frontalis cortex,ventral tegmental area was observed and the results were analyzed by image analysis system.Results Immunoreactivity for PSD-95 had statistical difference between the model groups and the control group(P0.01).It was strong in hippocampus and neostriatum in rats of dependent 1 week,and declined in 2 weeks,then increased in 4 weeks,but was increased in 1 week then declined in 2 weeks up to 4 weeks in amygdaloid body and frontalis cortex and ventral tegmental area.Results were highly singnificant difference in multiple comparison(P0.01).Conclusion There are significant changes of PSD-95 immnnoreactivity in withdrawal rats during the different dependent time.This indicate that PSD-95 has take part in morphine withdrawal memory in some cerebral regions.