Kinetic Expression of Thioredoxin in Lung Injury of Premature Rats Exposed to Hyperoxia and Its Clinical Significance

Objective To investigate the changes and potential role of the expression of thioredoxin(Trx) in lung of premature rats exposed to hyperoxia and its clinical significance.Methods In the first day after delivery,128 preterm SD rats were randomly divided into air group(n=64) and hyperoxia group(n=64).The rats were respectively killed in d1,4,7,10,14.Total RNA of lung was isolated and the expression of Trx mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR).Immunohistochemistry was used on lung sections to detect the expression of Caspase-3.The sections of lung were stained with HE in order to assess lung histologic changes.Results Rats in hyperoxia group showed typical lung injury,which was characterized by delay of lung development.Compared with air group,Trx mRNA expression in hyperoxia group markedly increased,peaking at d10(P0.05,0.01).Expression of Caspase-3 increased in hypero-xia group as well(P0.05,0.01).Conclusion Trx mRNA expression increasing can be induced by hyperoxia,which might play importantly protective role in development of hyperoxia-induced lung injury.