2010•Journal of Ningxia Medical UniversityRequires access

BDV Naturally Infected in Brain Tissue of Sheep in Ningxia

Zhenhai Wang

Abstract

Objective To investigate BDV naturally infected in brain tissue of sheep in Ningxia,and to explore the homology with the German horse-borne standard strain He/80.Methods BDV p24 gene and BDV p40 in brain tissue of 163 sheep cases in Ningxia were detected with Fluorescence quantitative nested reverse transcriptase PCR(FQ-nRT-PCR) and RT-PCR detection.The BDV p24 positive samples were sequenced and compared with the foreign standard virus strains.Results The positive rate was 3.68%(6/163) in brain tissue of sheep.after sequence comparison,BDV p24 fragment of the nucleotide sequence and He/80 showed 100% homology.Conclusion The brain tissue of sheep of Ningxia may exist latent infection of BDV and the positive BDV p24 nucleotide sequence is highly homologous with He/80.

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Key concepts: Brain tissue, Homology (biology), Virology, Biology, Nucleic acid sequence, Reverse transcriptase, Sequence homology, Nucleotide