Expression of Human Interleukin 10 in Pichia pastoris

The gene encoding human interleukin 10 was amplified with PCR from the pcDNA/3.1 plasmid, and then was cloned into the yeast expression vector pPIC9K containing the AOX1 promoter and the secretion signal sequence coding αfactor prepro-leader peptide. Then the yeast expression vector pPIC9K/hIL10 was constructed and transformed to P. pastoris GS115 strains, screened for multiple inserts, induced with0.5% methanol in shake flask cultures. Increasing level of rhIL10 was detected in the medium for up to 4 days by SDS-PAGE analysis. Western blot shows that the expressed rhIL10 exhibits high specificity and antigenicity.