Comparison of different doses intravenous injection of bleomycin-induced pulmonary fibrosis in mice

Objective To establish a mouse model of pulmonary fibrosis by using an improved program.Methods C57 / BL6 male mice were randomly divided into the single-dose group and the multiple-dose group. The single-dose group were randomly divided into the groups of 100,150,200 mg / kg and the control group 1. The multiple-dose group were randomly divided into the 50 mg / kg group and the control group 2. Their pathology of each lungs,immunohistochemistry of Col-Ⅰ,TGF-β1 and α-SMA were analyzed. Results The level of alveolitis reached the highest point at the second week in the single-dose group( P 0. 01),and the level of pulmonary fibrosis reached the highest at the second and fourth weeks,respectively( P 0. 01). In the 50 mg / kg group,the levels of alveolitis and fibrosis peaked at the 10 th week( P 0. 05). TGF-β1 and α-SMA increased significantly in parts of alveolitis and pulmonary fibrosis. Conclusion In an intravenous delivery,a single dose of 200 mg / kg bleomycin can effectively reproduce pulmonary fibrosis.