The Damage of Retinal Ganglion Cells in Chronic High Intraocular Pressure Mice

Objective: By burning the sclera venous to establish the chronic high intraocular pressure models,we studied the apoptosis of retinal ganglion cells in chronic high intraocular pressure mice.Methods: Take C57BL/6J mice 60,3 mice as blank control group,and the right eyes and left eyes of other 27 mice were used as the experiment group and the control group,respectively.The preoperative intraocular pressure was measured by iCare tonometer.The chronic high intraocular pressure models were established by burning the sclera venous.The postoperative intraocular pressure was measured by iCare tonometer everyday.Selected blank control group 6 eyes and each of the successful model of chronic high intraocular pressure 16 eyes(1 w,4 w after sclera venous burn),And then producing the paraffin sections of mouse eye.Tunel method was used to observe the apoptosis of retinal ganglion cells.Image was acquired under the fluorescence microscopy.The intraocular pressure was analyzed by t-test.Results: After sclera venous burn(1 d,1 w,4w),there were significant different(t=10.77～18.08,P0.001) in the intraocular pressure between the experimental groups and control groups(11.15 ±0.98,10.65±0.95,10.35±1.05 and 6.40±0.95,6.35±1.05,6.50±1.15).Tunel method showed no apoptosis of retinal ganglion cells in blank control group of normal mice.After sclera venous burn(1 w,4w),the chronic high intraocular pressure group were visible Tunel positive expression.But the control group had no Tunel positive expression.Conclusion: Burning the sclera venous induces durative and affirmative intraocular hypertension in mice.The apoptosis of retinal ganglion cells appeared with the extension of time after chronic high intraocular pressure model was established.And apoptosis is a main form of death of retinal ganglion cells in experimental high intraocular pressure in mice.