Inhibition of proliferation and induction of apoptosis by proteasome inhibitor MG-132 on C6 glioma cell in vitro

Objective To investigate the inhibition of proliferation and induction of apoptosis by proteasome inhibitor MG-132 on C6 glioma cell in vitro. Methods Rat glioma C6 cells were cultured with different concentrations of proteasome inhibitor MG-132 (10,20 and 50 μmol·L-1).Cell viability was determined by MTT assay at different cultured periods.Flow cytometry was used to detect apoptosis change and HE and AO/EB staining and electronic microscope were used to detect the morphological changes of apoptotic cells.Results Compared with normal control,MG-132 significantly reduced the viability of C6 cells in 10,20 and 50 μmol·L-1MG-132 groups (P0.01).Moreover,the inhibitory effect was higher on high concentration MG-132 compared with low dose MG-132. After treatment with 10 μmol·L-1MG-132 for 24 h,the apoptosis characteristic C6 cells were detected by AO/EB and HE staining.Apoptotic sub-G was detected by flow cytometry in C6 cells incubated with 10 μmol·L-1 MG-132 for 12 h and displayed a time-dependent manner,the apoptotic percentages in 10,20 and 50 μmol·L-1 MG-132 groups were significantly higher than that in control group (P0.01).Conclusion Proteasome inhibitor MG-132 can inhibit C6 cell proliferation and induce C6 cell apoptosis.