2006WIT transactions on ecology and the environmentRequires access

Medium option for Microcystis aeruginosa、Scenedesmus quadricauda and Cyclotella in competiton experimentations

Jiang Xia

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Abstract

Growth behaviors in pure culture of Microcystis aeruginosa,Scenedesmus quadricauda and Cyclotella were discussed through changing nitrogen forms and carbon concentrations in this article.Then one sort of fitting medium was selected as a multi-medium.It is reported that the max biomass and max specific growth rate of Microcystis aeruginosa and Scenedesmus quadricauda in ammonium medium were lower than in nitrate medium;It is proved that ammonium absorption ability of Microcystis aeruginosa and Scenedesmus quadricauda was improved by adding high HCO3-(NaHCO3 1.410 mmol/l)concentration.Original specific growth rate and Original biomass of Scenedesmus quadricauda were largely higher than Microcystis aeruginosa in low carbon(NaCO30.0943 mmol/l)concentration medium.But its max specific growth rate was lower than Microcystis aeruginosa.The bio-mass of Microcystis aeruginosa surpassed Scenedesmus quadricauda after the test had begun 10 days;Cyclotella could not survive in low carbon(NaHCO3 1.504 mmol/l) concentration mediaum;As a result,Microcystis aeruginosa,Scenedesmus quadricauda and Cyclotella can be pure cultured in ammonium(HA) or nitrate(HN) as nitrogen resource medium and their biomass have reached the quantity which experimentation needed.Therefore HN and HA are feasible multi-culture-media for this three algae in lab.

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Growth behaviors in pure culture of Microcystis aeruginosa,Scenedesmus quadricauda and Cyclotella were discussed through changing nitrogen forms and carbon concentrations in this article.Then one sort of fitting medium was selected as a multi-medium.It is reported that the max biomass and max specific growth rate of Microcystis aeruginosa and Scenedesmus quadricauda in ammonium medium were lower than in nitrate medium;It is proved that ammonium absorption ability of Microcystis aeruginosa and Scenedesmus quadricauda was improved by adding high HCO3-(NaHCO3 1.410 mmol/l)concentration.Original specific growth rate and Original biomass of Scenedesmus quadricauda were largely higher than Microcystis aeruginosa in low carbon(NaCO30.0943 mmol/l)concentration medium.But its max specific growth rate was lower than Microcystis aeruginosa.The bio-mass of Microcystis aeruginosa surpassed Scenedesmus quadricauda after the test had begun 10 days;Cyclotella could not survive in low carbon(NaHCO3 1.504 mmol/l) concentration mediaum;As a result,Microcystis aeruginosa,Scenedesmus quadricauda and Cyclotella can be pure cultured in ammonium(HA) or nitrate(HN) as nitrogen resource medium and their biomass have reached the quantity which experimentation needed.Therefore HN and HA are feasible multi-culture-media for this three algae in lab.

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Available abstract

Growth behaviors in pure culture of Microcystis aeruginosa,Scenedesmus quadricauda and Cyclotella were discussed through changing nitrogen forms and carbon concentrations in this article.Then one sort of fitting medium was selected as a multi-medium.It is reported that the max biomass and max specific growth rate of Microcystis aeruginosa and Scenedesmus quadricauda in ammonium medium were lower than in nitrate medium;It is proved that ammonium absorption ability of Microcystis aeruginosa and Scenedesmus quadricauda was improved by adding high HCO3-(NaHCO3 1.410 mmol/l)concentration.Original specific growth rate and Original biomass of Scenedesmus quadricauda were largely higher than Microcystis aeruginosa in low carbon(NaCO30.0943 mmol/l)concentration medium.But its max specific growth rate was lower than Microcystis aeruginosa.The bio-mass of Microcystis aeruginosa surpassed Scenedesmus quadricauda after the test had begun 10 days;Cyclotella could not survive in low carbon(NaHCO3 1.504 mmol/l) concentration mediaum;As a result,Microcystis aeruginosa,Scenedesmus quadricauda and Cyclotella can be pure cultured in ammonium(HA) or nitrate(HN) as nitrogen resource medium and their biomass have reached the quantity which experimentation needed.Therefore HN and HA are feasible multi-culture-media for this three algae in lab.

Key concepts: Microcystis aeruginosa, Scenedesmus, Microcystis, Ammonium, Biomass (ecology), Botany, Nitrate, Nitrogen

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