Cloning and Sequencing of the Varibale Region's Gene Fragments of the Anti-FMDV Monoclonal Antibody 1C7

The V H and V L genes were amplified from a hybridoma cell line 1C7 producing mouse McAb against foot-and-mouth disease virus by RT-PCR.The results of sequence determination showed that PCR products of V H region consisted of 368 bp encoding about 122 amino acid resides,and PCR products of V L region contained 323 bp encoding about 107 amino acid resides.The sequences of V H and V L genes were homologous with those of mouse antibody variable region published in NCBI Genbank.According to Kabat classified method,mAb 1C7 V H and V L gene segments belong to the mouse Ig heavy chain subgroup VH 7183 and K chain subgroup V K 20 respectively.1C7 V H was constructed by the rearrangement of VH76-1BG-DFL16.1-JH4 and 1C7 V L by the rearrangement of V K bw20-JK2.The success of cloning of the gene fragments of mAb 1C7's variable regions laid a benefit basis for the construction and expression of anti-FMDV single-chain Fv.