A Study on the Difference of Efflux Protein Expression between Klebsiella Pneumoniae Used in Vitro Fluoroquinolone and Ones Not Used

Objective To study the difference of efflux protein expression between klebsiella pneumoniae used in vitro fluoroquinolone and ones not used. Methods 20 sensitive klebsiella pneumoniae isolated from patients were divided into 2 groups .One group, 10 bacteria, were acted as contrast group. The other group, induced by different-density ciproxacin, and changed into resistant-ciproxacin klebsiella pneumoniae, were acted as survey group. Then monitor the difference of Minimum Inhibitory Concentration (MIC) according to using the inhibitor of efflux pump and expressing outer-membrane protein TolC between two groups. Results Induced by ciproxacin of from low to high density, sensitive clinical klebsiella pneumoniae were turned to high-resistance bacteria and outer-membrane protein TolC,53KDa, increased significantly. MIC was decreased by inhibitory of efflux pump in 70% resistant bacteria. Conclusions In vitro fluoroquinolone use can lead to the appearance of resistant klebsiella pneumoniae. The TolC increase indicate that efflux mechanism plays an important role in resistant bacteria. The inhibitor of efflux pump may some repress bacterium resistance.