Immobilized laccase and its properties by metal organic framework MOF-199

The immobilization of laccase and the properties of the immobilization laccase were studied.Laccase was immobilized by metal-organic framework MOF-199 as the carrier and 3-aminopropyltriethoxysilane was modified on the surface of the carrier,then glutaraldehyde as crosslinking agent.The laccase immobilized on MOF-199 were optimized to achieve the most optimal conditions of immobilization.The results showed that: laccase concentration was 0.3 g/L,load of glutaraldehyde was 1%(V/V),the value of pH is 4.8,and the cross-linking time is 7 h.Results showed that the immobilized laccase optimum temperature is 40 ℃,the optimum pH is 5.2,after 7 times in continuous operation,the enzyme activity still remained at 51%,the thermal stability of immobilized laccase,pH tolerance,storage stability were higher than that of the free laccase.