Introduction of Mechanism on Human Gastric Cancer Cell Line BGC-823 Growth and Inducing Apoptosis

Objective:The study was aimed to investigate the effect and mechanism of curcumin induced apoptosis on human gastric cancer cell line BGC-823.Methods:Cultured BGC-823 cells were divided into control,lower dose curcumin,middle dose curcumin and higher dose curcumin groups,and concentration of curcumin of each group were 0mg/L,5mg/L,10mg/L,20mg/L respectively.After pretreated with curcumin for 24h,the proliferation level was measured by MTT assay,cell apoptosis level was detected with flow cytometr.Whilst Bax、Bcl-2 protein expression levels were tested by immunohistochemisty assay and Caspase-3 mRNA expression level was tested by RT-PCR.Results:MTT assay showed the inhibitory effect of curcumin on proliferation of BGC-823 cells was dose dependent.Treatment with increasing dose of curcumin in the cells caused a dose-dependent apoptosis by flow cytometry analysis,such as 20mg/L curcumin pretreatment cause 48.3% cells apoptosis.Immunohistochemisty assay data indicated that Bax expression level was enhanced in curcumin treatment BGC-823 cells,and Bcl-2 protein expression level was repressed.And RT-PCR indicated that Caspase-3 mRNA expression was induced by curcumin.Conclusion:The promotion effect of cucumin on apoptosis of BGC-823 cells was dose dependent,which correlated with Bax up-regulation as well as Bcl-2 down-regulation and Caspase-3 activation signal transduction pathway.The data offer the clue for further understanding the inhibition mechanism of cucumin on human gastric cancer cell line BGC-823.