Protective Effects of Seven Kinds of China Traditional Medicine on Ethanol-induced Injury in PC12 Cells

Objective To observe the protective effect of Radix Paeoniae Rubra,stir-baked Radix Paeoniae Alba,Salvia Miltiorrhiza,Panax Notoginseng,Sanguisorba Officinalis,Radix Astragali,and Angelica Dahurica on ethanol-induced injury in pheochromocytoma(PC12) cells.MethodsDifferent doses of China traditional medicine were added to cultivate PC12 cells,and 2h later,ethanol 800 mmol/L was added and then coincubated for 24 h.The cell viability was assessed by MTT assay.Results Compared with the group treated by ethanol alone,the survival rates of Radix Paeoniae Rubra treated group(2.0-10.0 mg/ml),Sanguisorba Officinalis treated group(0.125-14.0 μg /mL) and Salvia Miltiorrhiza treated group(8.0-20.0μg /ml) was significantly higher,respectively(P0.01).Among these traditional Chinese medicines,10 mg/ml Radix Paeoniae Rubra,2 μg /ml Salvia Miltiorrhiza and 14 μg /ml of Sanguisorba Officinalis showed the most potent protective effects to ethanol-induced injury in PC12 cells.Stir-Baked Radix Paeoniae Alba,Panax Notoginseng,Radix Astragali and Angelica Dahurica did not show protective effects to ethanol-induced injury in PC12 cells.Conclusion Radix Paeoniae Rubra,Sanguisorba Officinalis and Salvia Miltiorrhiza have the effective protection against ethanol-induced injury in PC12 cells.