RESEARCH OF SEPARATION OF ENANTIOMERS OF DRUGS BY CAPILLARY ELECTROPHORESIS WITH CYCLODEXTRIN AS CHIRAL SOLVATING AGENT

Some basal research was done in capillary electrophoresis for separation of ten enantiomeric pairs of marketed drugs upon addition of β-cyclodextrin (β-CD) or hydroxypropyl-β-cyclodextrin (HP-β-CD,the substitution degree is 5.5) or their mixture to the run buffer which contained 40 mmol/L sodium dihydrogenphosphate and was adjusted to pH 2.5.The action about enantiomers entrapped in the matrix and the forming action of the inclusion-complexation between enantiomers and cyclodextrin were mainly dicussed.Moreover,we elaborated the significance of side-on-binding versus inclusion in the cavity of the cyclodextrin and the secondary hydroxyl groups acted the most important role.