Y chromosome microdeletion in male nonidiopathic infertility population from South Anhui province

Objective:To explore the relationship between Y chromosome microdeletion and non-idiopathic infertility in male cases with dyszoospermia.Methods:Multiplex PCR was used to detect the samples from 86 patients with this disease,among whom 61 were azoospermia and 25 were oligospermia,for screening the 6 STS loci from AZFa,b and c regions.10 male subjects in normal reproductive state were recruited as controls for the comparison of Y chromosome microdeletion.Results:Y chromosome microdeletion was found in 7(8.1%) of 86 non-idiopathic infertile males of dyszoospermia(50 cases with varicocele,27 with history of mumps and 9 with cryptorchidism).Microdeletion in region AZFb was seen in 2 cases,AZFc in 3 and both AZFb and c in 2,of the total 7 cases.Deletion was not screened in AZFa region,and no deletion was found in the 10 controls.Conclusion:Screening Y chromosome microdeletion should be recommended in male nonidiopathic infertility patients with dyszoospermia for the evidence of diagnosis and treatment in terms of genetics.