2009•International Journal of Digestive DiseasesRequires access
Experimental study on the change of intestinal immune function in rats with hyperlipidemia and severe acute pancreatitis
Lin Yang
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Abstract
Objective To explore the change of intestinal immune function in severe acute pancreatitis in rats with and without hyperlipidemia and its possible mechanisms.Methods A total of 40 male SD rats were randomly divided into 4 groups:group A(control group),group B(group of hyperlipemia),group C(group of SAP),group D(group of hyperlipemia+SAP).Hyperlipemia rat model was established by feeding a high-fat diet for 4 weeks.SAP rat model was established by retrograde injection of 3.5% sodium taurocholate into the biliopancreatic duct.Rats were sacrificed at 24 hours after models were made;the concentration of TG in serum was measured by clinical automatic biochemical analyzer,endotoxin concentration in portal vein was determined by limulus methods;CD4+,CD8+T lymphocytes in intestinal mucosa were examined by immunohistochemistry;the sIgA contents of intestinal mucosa was examined by radioimmunoassay;the glutamine uptake rate,concentration of glutamine and activity of GA in intestinal mucosa was examined by using spectrophotometry.Results The serum TG value in the group B was higher than that in the group A(P0.01).The serum TG value in the group D was higher than that in the group C(P0.01).Compared to the group A,plasma endotoxin concentration in the portal vein increased,and the levels of CD4+,CD8+T lymphocyte,sIgA,glutamine uptake rate,glutamine and activity of GA in the intestinal mucosa reduced significantly in the group C(P0.05～P0.01);Compared to the group C,the group D had a higher level of plasma endotoxin concentration in the portal vein and lower levels of CD4+,CD8+T lymphocyte,sIgA,glutamine uptake rate,glutamine and activity of GA in the intestinal mucosa(P0.05～P0.01).Conclusions Intestinal immune suppression occurred in the early stage of SAP.Hyperlipemia can make SAP more serious through intestinal immune suppression way,and the change of glutamine metabolism in the intestinal mucosa may play an important role.