Effects of deleted in liver cancer 1 gene transfection on the proliferation and migration of human ovarian cancer cell line OVCAR-3

Objective:To investigate the effects of deleted in liver cancer 1(DLC1) gene on the proliferation and migration of human ovarian cancer cell line OVCAR-3.Methods:The recombinant plasmid pEGFP-C3-DLC1 was transfected into OVCAR-3 cells by lipofectAMINE.The expressions of DLC1 mRNA and protein before and after transfection were detected by reverse transcriptase-polymerase chain reaction(RT-PCR) and Western blotting,respectively.The proliferation,cell cycle and migration ability of OVCAR-3 cells were determined by methyl thiazolyl tetrazolium(MTT) assay,flow cytometry and Transwell chamber assays in vitro,respectively.The protein expressions of focal adhesion kinase(FAK),c-Jun NH2-terminalprotein kinase(JNK),phospho-FAK(p-FAK)-Y925 and phospho-JNK(p-JNK)-Thr183/Tyr185 were detected by Western blotting.Results:Stable expressions of DLC1 mRNA and protein were observed in OVCAR-3 cells after transfection with DLC1 gene.The proliferation and migration abilities of OVCAR-3 cells were significantly reduced after transfection with DLC1 gene,and the cell cycle was arrested at G0/G1 phase(P0.01).The protein expressions of p-FAK(Y925) and p-JNK(Thr183/Tyr185) were markedly decreased in OVCAR-3 cells after transfection with DLC1 gene(P0.01).Conclusion:DLC1 gene can inhibit the proliferation and migration abilities of ovarian cancer cells through down-regulation of p-FAK(Y925) and p-JNK(Thr183/Tyr185) proteins,which leads to G0/G1 cell cycle arrest.