2010•Unpublished venueRequires access

Optimization of HPLC method for rapid determination of food additives

QI Yan-fei

Abstract

A high performance liquid chromatographic(HPLC)method for the rapid and simultaneous determination of the benzoic acid,sorbic acid and saccharin sodium was developed.The best separation was achieved with analytical column C18 column,250×4.6 mm,5 μm,by isocratic elution with mobile phase methanol/acetic acid(39/61,V/V),flow rate 1.0 mL/min,column temperature 35 ℃,UV diode array detection at 230 nm.The sensitivity of the method was determined by calculating the limit of detection(LOD)for each sample.The peak area of each pesticide was also determined.

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Key concepts: Chromatography, Sorbic acid, Chemistry, Detection limit, High-performance liquid chromatography, Benzoic acid, Methanol, Acetic acid