The Study of Dot Enzyme Linked Immunosorbent Assay for Clostridium perfringens Toxins

A Dot ELISA for detecting and identifying the α,β and e toxins of Clostridium perfringens were developed using the specific sheep anti Cl.perfringens α toxin IgG,specific sheep anti\|β toxin IgG and Mab against Cl.perfringens e toxin.Cultures filtrates of 8 type cultures of Cl.perfringens and samples of intestinal contents from 8 sheeps without Clostridium infection mixed artificialy with supernatants of cultures of different strains of Cl.perfringens were assayed one by one with the Dot\|ELISA,mouse toxicity assay and mouse neutralizing test.All results of these three assays were identical.Identical results were also obtained from another 10 field faeccal samples collected from a Clostridium disease free farm assayed with these three methods.Toxins as little as 0.001 MLD were detected by Dot\|ELISA.All of these works demonstrated that the Dot\|ELISA is a simpler easier and more specific method for detecting and identifying the Cl.perfringes toxins.