Value of combined detection of AFP mRNA and h-TERT mRNA in peripheral blood of hepatocellular carcinoma

Objective To study the value of combined detection of the serum levels of AFP mRNA and h-TERT mRNA in the early diagnosis of hepatocellular carcinoma.Methods The serum levels of AFP mRNA and h-TERT mRNA in 30 patients with hepatocellular carcinoma and 35 controls were measured by real-time quantitative PCR,then the correlation between the expression of h-TERT mRNA,AFP mRNA and the malignant nature of HCC was analyzed.Results AFP mRNA,h-TERT mRNA expression value in peripheral blood of patients with hepatocellular carcinoma were higher than the control group significantly(P0.01).The positive rate of AFP mRNA,h-TERT mRNA in hepatocellular carcinoma group were 76.7%,83.3%,respectively.They were significantly higher than those in control group(P0.01).But there were no expression in control group in AFP mRNA and there was appostive case in h-TERT mRNA in control group,Sensitivity of combined detection of two kinds of tumormarkers was 92.6%,The specificity of this experiment was 97.2 %.It was significantly higher than that of single detection.Conclusion Combination of detection of AFP mRNA、h-TERT mRNA in peripheral blood is a sensitive and valuable assay for the early prediction of the prognosis of the HCC patients.And it can also help to distinguish the potential patients.