2006•Journal of Traditional Chinese OphthalmologyRequires access
Effect of quzhangling(祛障灵)on apoptosis for expression of rat lens epithelial cells for Bcl-2 and Bax
Yinjian Zhang
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Abstract
OBJECTIVE To investigate effect of quzhangling(祛障灵)on apoptosis for expression of rat lens epithelial cells for Bcl-2 and Bax in rat lens epithelial cells(LEC)injured by oxidation.METHODS The model of oxidative damages were established in lens of rats and they were divided randomly into control group,Hydrogen peroxide group(H_2O_2),pirenoxine sodium group,quzhangling sodium,low dosage group and high dosage group.The expression of Bcl-2 and Bax protein of LEC were measured and compared by tearing the LEC anterior capsule via immunohistochemical analysis.RESULTS There were Bcl-2 and Bax expression in normal lenses of SD rates,Bcl-2 expression was stronger than Bax.Bcl-2 expression decreased and Bax expression increased markedly,Bcl-2/Bax reduced in H_2O_2 group.There were up regulation of Bcl-2 expression and down regulation of Bax expression,and Bcl-2/Bax increased in quzhangling group compared with H_2O_2 group.The above changes were similar between pirenoxine sodium group and quzhangling sodium group.CONCLUSIONS These results show that quzhangling regulates expression of apoptosis-related genes,Bcl-2 and Bax,which may be the molecular mechanism of LEC apoptosis inhibition by quzhangling.