Cultivation,Differentiation and Identification of Human Omental Adipose Derived Mesenchymal Stem Cells in Vitro

Objective To establish the primary culture and induced differentiation to fat cells of human omental adipose derived mesechymal stem cells(ADSCs).Methods The omental adipose tissues were obtained by opration and fibroblast-like cells was primarily cultured by collagenase digestion.The ADSCs were identificated by detecting the expression of CD44,CD34,CD90 and CD45 with flow cytometry,cell growth curve was measured by MTT method,the P3 cells were induced to fat cells with 3-isobutyl-1-methylxanthine,insulin,dexamethasone and indomethacin,and were identificated by oil red O.Results The cultured cells were homogeneous spindle-shaped or swirling and were identificated as ADSCs by flow cytometry.Cell growth curve showed the cells proliferated well.After induction with differentiation medium,cell became round,cell volume increased,fat particles aggregated in the cytoplasm,and the cells were identificated as mature fat cells by oil red O staning.Conclusion This method can culture homogeneous ADSCs,and ADSCs can differentiate into mature adipocytes after induction.