Antifibrotic mechanism of total flavone from Litchi chinensis Sonn in cholestatic liver fibrosis rats

Objective To investigate the function and mechanisim of total flavone from Litchi chinensis Sonn on collagen in cholestatic liver fibrosis rats. Methods The rats model of after ligation of the common bile duct into the common bile duct ligation( BDL group),Litchi flavonoids group( TFL group),silibinin group( SIL group),to the control group as a negative control. After four weeks,serum detection of serum alanine aminotransferase( ALT),aspartate aminotransferase( AST),direct bilirubin( BILD2),total bilirubin( BILT2),creatinine( CREJ2),blood urea nitrogen( UREAL) were measured,and the pathological observation was also performed on legt liver lobes with HE,Masson staining,western blot were measured in liver tissue PC3,PC Ⅰ,p16 expression. Results The serum SOD in TFL was significantly higher than that in the BDL group( P 0. 05),The serum ALT,AST,BILD2,BILT2 levels were significantly lower than that in the BDL group( P 0. 05),TFL can significantly improve the degree of hepatic fibrosis in rats( P 0. 05),and no significant side effect on renal function( P 0. 05),TFL can inhibit p16,PC3,PC Ⅰprotein expression( P 0. 05). Conclusions TFL group( 300mg /kg·d) has a significant improvement in cholestasis and liver fibrosis mitigate and had no significant side effect on renal function,significantly inhibited cholestatic liver fibrosis in rat liver p16,PC3,PC Ⅰ protein,probably by inducing apoptosis pathway inhibition of hepatic fibrosis.