Study on Embryo Transfer Technology in Kunming Mouse

Objective The aim of the study were to investigate the influencing factors about the success rates of embryo transfer at diffferent periods and improve the successful rates of embryo transfer.Methods KM female mouse with superovulation treatment were mated with these normal 615 male mouse,fertilized eggs are obtained.Then these 1-cell embryo,2-cell embryo,morula and blastocyst were respectively transferred into the oviduct and the uterus of pseudopregnancy KM mouse.Results Inflowed 1-cell embryo,2-cell embryo,morula and blastocyst,pregnant rates of the KM mouse were respectively 36.36%,42.86%,10% and 27.27%,farrowing rates are respectively 46.58%,38.46%,58.33% and 60%.Conclusion 2-cell embryo transplantation in KM mouse can improve the success rate of mouse embryo transfer,uterus transplantion can improve the farrowing rates of mouse embryo transfer.