2004•Zhiwu yanjiuRequires access
Cloning of inducible promoter rd29A and the construction of plant express vector
Jing Li
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Abstract
According to the reported sequence of inducible expression promoter rd29A a pair of primer was designed and synthesized. Using the genomic DNA of Arabdopsis thaliana as templet, fragment of rd29A promoter was amplified through method of PCR. According to the cDNA sequence of transcription factor DREB1A gene in GenBank a pair of primer was designed and synthesized. Using the total RNA of low temperature treated Arabdopsis thaliana seedling as templet, the full length of cDNA of DREB1A gene was amplified through method of RT-PCR. The plant express vector pBch was digested and ligated with the DREB1A cDNA fragment and rd29A promoter fragment and got the DREB1A gene plant express vector pBDR29A regulated by inducible promoter.