EFFECTS OF CORD BLOOD SERUM ON COLONY CULTURE OF BONE MARROW HEMATOPOIETIC PROGENITOR CELLS

Aim: To provide experimental proofs for Cord Blood clinical appliance, the effects of human CBS (Cord Blood Serum) on colony culture of bone marrow hematopoietic progenitor cells were studied. Methods: Observed the colony forming capacity of CFU GM,CFU E,BFU E,CFU GEMM when they were cultivated using hBMNC with CBS, Mixed Cord Blood Serum (MCBS) and/or cytokines. Resutts: 10% CBS could promote the forming of CFU GM of BMC directly, no matter what blood group the CBS or BMC were of. If four or more copies of CBS were mixed (MCBS), no matter whether the “ABO” blood groups between them were same or not, they could produce more CFU GM than single copy or two copies mixed CBS when used, and their activity was stable. MCBS could increase the production of CFU GM、CFU E、BFU E、CFU GEMM in routine culture system, and 10%MCBS corresponds with 65.6 μg/L GM CSF,0.23,0. 3,0.46 kU/L Epo to CFU GM,CFU E,BFU E,CFU GEMM respectively. When used in colony culture of CFU GM,20% MCBS has the same effect as 100 μg/L GM CSF with 20% HS. 20% MCBS could also directly promote the forming of CFU E. It formed few or none BFU E and CFU GEMM without BpA and some BFU E and CFU GEMM when with BpA. Conclusion: ①SCBS (MCBS) contains such substances as GM CSF, EPO, and so on. ②There exists some activities which can promote the forming of CFU GM, CFU E, BFU E, CFU GEMM in CBS (MCBS). ③These activities would be stronger and more stable if four or more copies of any blood group CBS were mixed. It may be a fine prospects of clinical appliance.