Study on the Effect of TGF-β1 on Biliary Anastomatic Stricture

Objective:To explore the effect of TGF-β1 on pathologic procedure of biliary anastomotic stricture.Methods: Twemtu-four experimental Bama mini-pigs were randomly divided into three groups: the sham operation control group(Group C),we stabbed(not actually anastomosed) the bile duct by anastomotic needle after dissecting the bile duct;The operation control group(Group S),we excised the bile duct 5mm in length after dissecting the bile duct and treated by end-to-end anastomosis;The treatment group(Group T),we treated in the same way as the Group C and drained the bile by inserting a Forley urethral catheter in the bile duct above the anastomotic stoma 15mm,then infused water into the air chamber(the amount was determined by the diameter of the bile duct) to block the bile flow passing the anastomotic stoma.Then we analyzed the degree of hyperplasia in each group,the ratio of the diameter of the anastomotic stoma to the bile duct above,and the expression of TGF-β1 by immunohistochemistry method.Results: There were anastomotic stoma hyperplasia and decrease in diameter in Group C,Group S and GroupT,the lenghth of the wall of the bile duct were 0.9±0.2mm,1.7±0.2mm and 1.0±0.3mm,respectively.When compared Group T with Group C,the result was not statistically significant(P0.05).When compared Group S with Group T and Group C,the results were statistically significant(P0.05).The ratio of the diameter of Group C,Group T and Group S was 0.96±0.13,0.89±0.15 and 0.68±0.17,respectively.When compared Group S with Group C and Group T,the results were statistically significant(P0.05).There were expression of TGF-β1 differently in GroupC,Group S and GroupT,the positive expression cells was 13,37 and 16 respectively.When compared Group S with Group C and Group T the results were statistically significant(P0.05).Conclusions: TGF-β1 takes part in pathologic procedure of biliary anastomotic stricture.Its expression degree follows biliary anastomatic stricture.But what role it plays in biliary anastomotic stoma stricture maintains unknown.