Effect of Fangjihuangqi Decoction on Proliferation of Hepatic Stellate Cells in Lipopolysaccharide-intervented Rats

Objective:To evaluate the effect of Fangjihuangqi decoction on proliferation of hepatic stellate cells(HSC) in rats with lipopolysaccharide(LPS) intervention.Methods:HSC-T6 cells were cultured with high glucose DMEM(containing 10% FBS) with 1 EIU/mL LPS.The cells were then divided into 5 groups:blank control group,LPS group,and low-,medium-,and high-dose of Fangjihuangqi decoction groups.MMT assay was used to consecutively determine the absorbance for 5 days.ELISA method was used to detect the secration of MMP-2,TIMP-1,and TGF-β1 in the HSC-cultured supernatant after 48 h.Results:Fangjihuangqi decoction inhibited the proliferation of HSC,more obvious in the high-dose group.Compared with the LPS group,the high-dose test drug significantly increased the level of MMP-2 and decreased the level of TIMP-1 and TGF-β1(P0.01).Conclusion:Fangjihuangqi decoction can inhibit the proliferation of HSC by upregulating MMP-2 and downregulating TIMP-1 and TGF-β1 in LPS-intervented rats.