Study on the Hemostatic Effect of the Extracts from Liparis nervosa

OBJECTIVE: To study the effect of Liparis nervosa on promoting hemostasis and blood coagulation. METHODS: The mice were given high-dose and low-dose(4,2 g/kg) aqueous extract, aether petrolei extract, dichloromethane extract, water extraction and alcohol precipitation of L. nervosa intragastrically once a day for consecutive 7 days. The bleeding time (BT) was determined by shearing tail method. Clotting time(CT) was detemined by capillary tube method. The rats were given high-dose and low-dose(4,2 g/kg) aqueous extract, aether petrolei extract,dichloromethane extract, water extraction and alcohol precipitation of L. nervosa intragastrically once a day for consecutive 7 days. The blood samples were collected from carotid artery for the determination of prothrombin time (PT),activated partial thromboplastin time(APTT),thrombin time(TT) and fibrinogen(FIB). RESUL- TS:High-dose and low-dose dichloromethane extract of L. nervosa could shorten BT of mice significantly(P0.05);CT and the APTT values were obviously shortened by low-dose dichloromethane extract(P0.05),and FIB values were obviously increased by all of the extracts of L. nervosa(P0.05). CONCLUSIONS:L. nervosa has an effect on promoting hemostasis and blood coagulation,and its mechanisms may be related with shrinking blood vessels, inhibiting plasmin activity and rising FIB content.