Analysis of Genetic Diversity among Barleys Came from 19 Asian Countries Using Fluorescent SSR Markers

Understanding the genetic diversity of barley germplasm could be the basis for appropriate selection of parents in breeding program,and could enhance protection and utilization of barley germplasm.In this study,genetic diversity analysis of 175 barely accessions from 19 Asian countries was analyzed by 21 combinations of fluorescent SSR primers.The polymorphic information content(PIC) and marker index(MI) were calculated for each SSR primer.According to the genetic similarity,the polygenetic tree of the genetic relationship among the 175 barley accessions was drawn.According to the Genetic identity(GI),the polygenetic tree and the charts of principle coordinates analysis of the genetic relationship in barley accessions from the populations of 19 countries were classified by origins.The results showed nineteen pairs of SSR primers amplified 134 allelic loci with 7.05 per SSR primer pairs among the 175 cultivars.The results indicated that locus SSR17 was effective to detect DNA variation among those barley accessions from Asian.The highest value of polymorphism information content(PIC) and marker index(MI) was 0.9 and 10.77,respectively.Based on the polymorphism data,175 barley accessions from 19 Asian countries were clustered into 3 groups.Group I contained 161 accessions from 19 countries,group II comprised 12 accessions from 4 countries,only 2 accessions from Turkmenistan were clustered into group Ⅲ.According to the Nei's genetic identity(GI),19 national populations were classified into 3 groups by UPGMA phylogenetic analysis,and group 1 was with 4 subgroups.By using principle coordinates analysis,these populations were clustered into 3 groups and 7 subgroups.Results indicated that the classification with the two methods was basically similar with identification of highly genetic diversity of Asian barley accessions,but the principle coordinates analysis could be better.