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2007•Beijing Medical JournalRequires access

Establishment of genotyping technique on ABO blood group in Beijing

Zhuo-Yan Wang

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Abstract

Objective To establish a diagnostic method for ABO genotyping and to provide a simple and reliable technique for safe transfusion and identification of complicated blood group. Methods ABO genotyping method was developed by using multiplex-PCR-RFLP and PCR-SSP techniques. Results We developed a set of methods, which were stable and accurate for ABO genotyping. This diagnostic system were excellent for ABO genotyping after implementation. Conclusions We have established an reliable ABO genotyping diagnostic system.

About this research paper

What this paper is about

Objective To establish a diagnostic method for ABO genotyping and to provide a simple and reliable technique for safe transfusion and identification of complicated blood group. Methods ABO genotyping method was developed by using multiplex-PCR-RFLP and PCR-SSP techniques. Results We developed a set of methods, which were stable and accurate for ABO genotyping. This diagnostic system were excellent for ABO genotyping after implementation. Conclusions We have established an reliable ABO genotyping diagnostic system.

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Method / approach

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Available abstract

Objective To establish a diagnostic method for ABO genotyping and to provide a simple and reliable technique for safe transfusion and identification of complicated blood group. Methods ABO genotyping method was developed by using multiplex-PCR-RFLP and PCR-SSP techniques. Results We developed a set of methods, which were stable and accurate for ABO genotyping. This diagnostic system were excellent for ABO genotyping after implementation. Conclusions We have established an reliable ABO genotyping diagnostic system.

Key concepts: Genotyping, ABO blood group system, Medicine, Multiplex, Multiplex polymerase chain reaction, Genotype, Polymerase chain reaction, Genetics

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